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Medium is used for sterility test. The sample or its suspension, bring in a sterile environment in test tubes and cultivated at 37 ° C for 24-48 hours. Left the second uninoculated vial as a control test. When microbiological pollution becomes cloudy, aerobic form blurred in the upper part of the tube, anaerobes at the bottom. A feature of the environment is the ease of analysis, and the ability to analyze aerobic and anaerobic microflora. This is achieved with agar-agar causes  semi-liquid properties of the medium, and thioglycolate sodium which maintains anaerobic conditions at the bottom of the tube.