Medium Olkenitskyi, also known as three-sugar agar with urea. The sterile molten medium was poured into vials so that when cold formed filled "style" and "school" in contact with the air. The color of the finished medium is red or orange. The sowing is carried out by puncturing the environment of the microbial loop to the bottom of the tube. Samples cultured at 37 ° C for 24 hours, after which evaluate the change in the appearance of the environment. It can be used to identify the 5 indicators:
Fermentation of glucose - yellow "column";
Fermentation of sucrose and / or lactose - yellow "school";
The formation of hydrogen sulphite - blackening;
Fermentation of urea - crimson color;
The formation of gas - bubbles in the thickness of the agar.