Muller-Kauffmann Broth, also known as Tetrathionate broth used for the enrichment and accumulation of Salmonella. In the prepared medium are added aqueous solutions of iodine and brilliant green. The mechanism of action of the medium associated with the interaction of sodium thiosulfate and iodine and formed tetrathionate sodium. Formed substance inhibits the growth of bacteria, except Salmonella and Proteus. Tetrathionate sodium sensitive to changes in pH, so medium included calcium carbonate, which supports the acid-alkaline balance.
A distinctive feature of this environment is a complete inhibition of E. coli, after 24 hours of incubation.
The medium is sensitive to overheating. Not be autoclaved. After finish, the chilled medium has added a solution of iodine and brilliant green. The cultivation is carried out at a temperature of 42 ° C for 18-24 hours.